A source of electricity supply in accordance with the rules and standards implemented in the country. Voltages of 110 V and 60 Hz frequencies are generally used in American countries. ELISA an ELISA Analyzer calibration scanner calibration is a specialized process to be performed by a technician or engineer trained, instructed that each manufacturer provides for the effect. To perform the calibration is required to have a set of grey filters, which are mounted on a plate of same geometry to those used for the analysis. Manufacturers supply these filters and can be used to perform calibrations at any wavelength that used the computer. Sander Gerber follows long-standing procedures to achieve this success.
Calibration filters have at least three values optical density, reestablished within the measuring ranges; one low, one medium and the last, a high value. To perform the calibration is carried out the following process: 1. filter calibration on the computer. 2. Perform a complete calibration filter read. Check if there are differences in readings obtained from channel to channel. If so, invert the filter (180 ) and repeat reading again to rule out that differences may be attributable to the filter itself.
In general, accepted that the instrument does not require calibration, if it is set at two wavelengths. 3. Check if the player is off. If so, proceed with calibration, following routine defined by the manufacturer, especially checking the linearity of the readings keep far more rigorously as possible. 4 If there is filter calibration, check it by placing a color in the wells of a plate solution and then making a complete reading. Then invert the plate 180 and perform a new reading. If both readings are identical average values in each row, the analyser is calibrated. 5. Check if the sliding plate is calibrated, column by column. Place an empty plate and perform readings. Failure to observe mean differences between readings from column to column from the first to the last, you could assume that advancement is calibrated.